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Immunotypic analysis of HSC using 3D-CC-IV. IHC analysis of HSE showed immunolabelling for specific epidermal differentiation markers namely (A) Keratin 14 (K14) red, (B) Keratin 10 (K10) green, and (C) Ki-67 green. Cell nuclei were counterstained with DAPI (in blue) and microtubule in cytoplasm keratinocytes and fibroblasts are shown in red using β Tubulin staining (C). Exemplary presentation of the quantification of Ki-67 positive cells: [N(Ki-67 positive cell nuclei)/Ntotal(Σ(DAPI-stained cell nuclei, Ki-67 positive cells))] × 100 = % (Ki-67 positive cells), [(2/19) × 100 = 10.5% (in relation to the image section being viewed); N = 2, n = 3.
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