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Macroscopic view of Human Dermal Construct (HDC) – upper panel: (A) View of experimental set-up of Human Dermal Construct on Day-01 with NHDFs are embedded in collagen I matrix and incubated to allow gel remodeling. (B) Macroscopic top view of contracted collagen on Day-07 retaining its circular shape. Macroscopic view of HSE – lower panel: (C) Top view of experimental set-up of HSE development during incubation at ALI showing the white epidermal layer over the dermal layer. (D) Side view of HSE containing insert at the time of tissue harvesting after 20 days at ALI, showing a thickness of ~2 mm. (E) Top view of harvested HSE at the end of ALI period (20 days) retaining the size and diameter of initial mould (12 mm in diameter).
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