Histological cross sections of HSC using the 3D culture conditions 3D-CC-I (upper panels 1A–C and 1A′–C′) and 3D–CC-II (lower panels 2A–D and 2A′–D′); N = 2, n = 3. Upper panels (1A, 1B, 1C) and (1A′, 1B′, 1C′) showed H&E-stained histological microscopy images of HSCs at different magnification elevated to the air-liquid interface (ALI) for 10 days and 15 days, respectively. Lower panels (2A, 2B, 2C, 2D) and (2A′, 2B′, 2C′, 2D′) show H&E-stained histological microscopy images of HSCs at different magnification elevated to the air-liquid interface (ALI) for 10 days and 15 days, respectively.