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Histological cross sections of HSC using the 3d culture conditions 3D-CC-IV; N = 2, n = 3. Upper (A, B, C, D), middle (A′, B′, C′, D′), and lower panels (A′′, B′′, C′′, D′′) (images at different magnifications) showed H&E-stained histological micrographs of HSCs raised for 10 days, 15 days, and 20 days at ALI, respectively. The longer the ALI cultivation period, the better a structured stratification of the epidermis from stratum basale to stratum corneum can be recognised (cf. labelling in Fig. 6).
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