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Table 2

Experimental set-up details of different 3D cell culture conditions (3D-CCs).

Step duration Description of 3D culture condition 3D-CC-I 3D-CC-II 3D-CC-III 3D-CC-IV
5–7 days HDC feeding FGM2 FGM2 CnT-PR-F (Medium A) CnT-PR-F (Medium A)
3–4 days Epidermal monolayer formation (onto HDC); feeding KGM2 KGM2 CnT-PR (Medium B) CnT-PR-FTAL
15–16 h Epidermal monolayer formation (onto HDC); feeding CnT-PR-3D (Medium C) CnT-PR-3D (Medium C)
10/15/20 days HSC culture at ALI for 10, 15, and/or 20 days 1.2 mM CaCl2 containing KGM2 CnT-PR-3D (Medium C) CnT-PR-3D (Medium C) CnT-PR-FTAL

Abbreviations: CnT-PR-F named as Medium A; CnT-PR named as Medium B; CnT-PR-3D named as Medium C.

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