Open Access
Table 2
Experimental set-up details of different 3D cell culture conditions (3D-CCs).
| Step duration | Description of 3D culture condition | 3D-CC-I | 3D-CC-II | 3D-CC-III | 3D-CC-IV |
|---|---|---|---|---|---|
| 5–7 days | HDC feeding | FGM2 | FGM2 | CnT-PR-F (Medium A) | CnT-PR-F (Medium A) |
| 3–4 days | Epidermal monolayer formation (onto HDC); feeding | KGM2 | KGM2 | CnT-PR (Medium B) | CnT-PR-FTAL |
| 15–16 h | Epidermal monolayer formation (onto HDC); feeding | – | CnT-PR-3D (Medium C) | CnT-PR-3D (Medium C) | – |
| 10/15/20 days | HSC culture at ALI for 10, 15, and/or 20 days | 1.2 mM CaCl2 containing KGM2 | CnT-PR-3D (Medium C) | CnT-PR-3D (Medium C) | CnT-PR-FTAL |
Abbreviations: CnT-PR-F named as Medium A; CnT-PR named as Medium B; CnT-PR-3D named as Medium C.