Experimental set-up details of different 3D cell culture conditions (3D-CCs).
|Step duration||Description of 3D culture condition||3D-CC-I||3D-CC-II||3D-CC-III||3D-CC-IV|
|5–7 days||HDC feeding||FGM2||FGM2||CnT-PR-F (Medium A)||CnT-PR-F (Medium A)|
|3–4 days||Epidermal monolayer formation (onto HDC); feeding||KGM2||KGM2||CnT-PR (Medium B)||CnT-PR-FTAL|
|15–16 h||Epidermal monolayer formation (onto HDC); feeding||–||CnT-PR-3D (Medium C)||CnT-PR-3D (Medium C)||–|
|10/15/20 days||HSC culture at ALI for 10, 15, and/or 20 days||1.2 mM CaCl2 containing KGM2||CnT-PR-3D (Medium C)||CnT-PR-3D (Medium C)||CnT-PR-FTAL|
Abbreviations: CnT-PR-F named as Medium A; CnT-PR named as Medium B; CnT-PR-3D named as Medium C.
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