Primary antibodies used for immunostaining of HSE tissue cryosections.
|Primary antibody details and source||Dilution||Clone-No.|
|Ki-67 (Rb, MA) – Cell signal||1:300||D2H10|
|Laminin 5 (Rb, PA) – Abcam||1:500||–|
|Keratin 14 (Ms, MA) – Abcam||1:100||LL002|
|Keratin 10 (Rb, MA) – Abcam||1:500||EP1607IHCY|
|Filaggrin (Rb, PA) – Abcam||1:200||–|
|β-Tubulin (Ms, MA) – Sigma||1:400||EPR16774|
|Loricrin (Rb, PA) – Abcam||1:200||–|
|Keratin 16 (Rb, PA) – Abcam||1:100 & 2° Ab as 1:200||–|
PA = polyclonal antibodies; MA = monoclonal antibodies; Rb = rabbit; Ms = mouse; 2° Ab = secondary antibodies.
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