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Table 3
Primary antibodies used for immunostaining of HSE tissue cryosections.
| Primary antibody details and source | Dilution | Clone-No. |
|---|---|---|
| Ki-67 (Rb, MA) – Cell signal | 1:300 | D2H10 |
| Laminin 5 (Rb, PA) – Abcam | 1:500 | – |
| Keratin 14 (Ms, MA) – Abcam | 1:100 | LL002 |
| Keratin 10 (Rb, MA) – Abcam | 1:500 | EP1607IHCY |
| Filaggrin (Rb, PA) – Abcam | 1:200 | – |
| β-Tubulin (Ms, MA) – Sigma | 1:400 | EPR16774 |
| Loricrin (Rb, PA) – Abcam | 1:200 | – |
| Keratin 16 (Rb, PA) – Abcam | 1:100 & 2° Ab as 1:200 | – |
PA = polyclonal antibodies; MA = monoclonal antibodies; Rb = rabbit; Ms = mouse; 2° Ab = secondary antibodies.