Open Access

Table 4

Details of reagents required for Immunostaining.

Reagent Type Reagent constitution Reagent Quantity (calculated)
Wash buffer TBS + 0.025% Triton X-100 250 µL (0.25 mL) Triton X-100 in 1000 mL TBS
Permeabilization reagent 0.2% Triton X-100 in PBS 20 µL (0.02 mL) Triton X-100 in 10 mL TBS
Fixative 4% paraformaldehyde in PBS 10 mL of 16% PFA in 30 mL PBS
Antibody diluent buffer TBS + 1% BSA 0.1 g BSA in 10 mL TBS
Blocking Buffer 10% normal serum + 1% BSA + TBS 1mL normal serum + 0.1 g BSA + 9 mL TBS
For double staining: 5% normal serum of species in which first secondary antibodies were raised + 5% normal serum of species in which second type of secondary antibodies was raised + 1% BSA + TBS
Note: Goat and donkey serum 60 mg/mL – Jackson Immuno Research
Buffers 1× TBS (10× TBS comprised of 500 mM tris base + 1.5 M NaCl + HCl to set pH to 7.4); 1× PBS 100 mL of 10× in 900 mL distilled deionized H2O

BSA is bovine serum albumin, TBS is tris-buffered saline, PBS is phosphate-buffered saline.

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.